Question

The following template sequence of DNA was isolated from a bacterial cell. Identify the promoter region (assume there is no operator) and transcribe the DNA to the right of the promoter. Once you’ve transcribed the DNA into mRNA, find the start codon and translate the sequence into the correct amino acids to create a polypeptide. 3’-AATATTTATTATATAACCCGGCATACGTTCGGACGTCCCATATAGACTCCATTCGACTT-5’

Answer 1

Template strand sequence:
3'-AATATTTATTATATAACCCGGCATACGTTCGGACGTCCCATATAGACTCCATTCGACTT-5'

Coding strand sequence:
5'-TTATAAATAATATATTGGGCCGTATGCAAGCCTGCAGGGTATATCTGAGGTAAGCTGAA-3'

Promoter sequence: 5'-TATAAA-3'

The TATA box is generally situated around 25-35 bp upstream of the transcription start site.
In this case, it is located 17 bp upstream to the translation start site.

The mRNA coding sequence: 5'-AUGCAAGCCUGCAGGGUAUAUCUGAGGUAA-3'

The amino acid sequence: NH2-Met-Gln-Ala-Cys-Arg-Val-Tyr-Leu-Arg-COOH