Question

You were given a sputum sample from a patient. Design an experiment on how you would undergo the identification of the possible microbiological agent. You can use any previous laboratories or even outside reliable references. Be sure to include any medias, testing, characteristics that would be useful. But before you are able to identify - what must to obtain from the sputum (hint some type of culture - but what type?

Answer 1

Answer:

• The sputum sample could be used for identification of the culture and the organism present in the sample.
• Sputum samples are usually tested to identify the infections associated with lower respiratory tracts.
• Sputum could be defined as the phlegm or the thick mucus that would be expelled from the lower portion of the respiratory tract.
• While collection, it Always has to be taken care that sample collection should be from the lower portion of the tract and not the upper areas of the tract.
• There would be pathogen detection by susceptibility trysting so that the antibiotic treatment could be guided.
• This is also done in the form of gram stain, for typical bacterial identification followed by nutrient media placement and incubation
• . There would be other tests such as fungal culture or afb smear or viral culture.

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