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Eukaryotic RNA viruses face problems in getting internal cistrons expressed. What are some ways that RNA...

Eukaryotic RNA viruses face problems in getting internal cistrons expressed. What are some ways that RNA viruses of eukaryotes have solved this problem? Using examples, describe the various strategies, the genome structures involved and how each strategy works.

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Answer #1

Eukaryotic RNA virus have difficult to express cistron because eukaryotic ribosome usually translate 5'cistron in a
polycistronic mRNA and cistron downstream of 5' cistron is effectively closed.

Two strategies to overcome this problem
1. All the genetic information in one open reading frame which is translated to give a polyprotein which later cleaved to functional protein.

2. The genoe is divided into monocistronic which can be translated individually. This division occurs either during transcription to give collection of 3' coterminal subgenome mRNA or multicomponent virus of RNA

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