Question

The EcoRI digest was performed to directly verify which of the following?

a. RNase successfully degraded any RNA in the sample

b. Genomic DNA was successfully isolated from the flies

c. The plasmids contained the inserts of interest for sequencing

d. The PCR primers used amplified a product of the expected size

Answer 1

Answer: EcoRI is a restriction enzyme that cuts the DNA at specific site called restriction sites (GAATTC) and forms fragments. EcoRI digest is used to digest the plasmid containing gene inserts of interest during cloning experiments. Before sequencing, the cloning of insert in to the plasmid is verified by restriction enzyme digestion. Hence, option c is the correct answer.

EcoRI is a DNase but not RNase and will not degrade RNA. Hence, option a is incorrect.

Sucessful isolation of genomic DNA from files and PCR amplification of DNA of expected size from the primers are verified by gel electrophoresis but not restriction emzyme digest. Hence, option b and d are incorrect.