Question

Among the following techniques, ________ is the method of choice to reveal the size of a mRNA transcript; ________ is the method of choice to detect the expression level of an extremely rare mRNA, ___________is the method of choice to detect the expression level of three genes with unknown nucleotide sequences, and ________ is the method of choice to quantitate the expression levels of >500 different genes in a single experiment.

(Write the correct answer in the blank)

           a. DNA microarray                  b. Real-time RT-PCR                         c. Northern Blotting

          d. in situ hybridization             e. next-generation sequencing            f. none of the above

Answer 1

Among the following techniques, NORTHERN BLOTTING/NEXT GENERATION SEQUENCING (RNA seq) is the method of choice to reveal the size of a mRNA transcript.

REAL-TIME RT-PCR is the method of choice to detect the expression level of an extremely rare mRNA.

NEXT GENERATION SEQUENCING (RNA seq) is the method of choice to detect the expression level of three genes with unknown nucleotide sequences.

DNA MICROARRAY is the method of choice to quantitate the expression levels of >500 different genes in a single experiment.