1)-Cells don't know that it needs more ATP. It is regulated by feedback system governed by cytosol level of e.g.AMP,ADP,ATP and Indirectly by several hormones.
2)-In glycolysis enzyme hexokinase, phosphofructokinase and pyruvate kinase are serve as control site.
3)-Allosteric effector of phosphofructokinase is based on cell's energy need. ATP act as allosteric inhibitor. Allosteric effector of pyruvate kinase is fructose 1,6-bisphosphate.
4)-NAD+ is oxidising agent and accept electron from other and reduced to NADH.
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