Question

Please type your answer WHICH COVERS BOTH PARTS

Colony characteristics Size • Shape • Color (also known as pigmentation) • Texture• Height (a.k.a. elevation) • Edge (a.k.a. margin) of Staphylococcus consisting of clustered cocci.

The microbe is originally isolated from skin infections among pigs and was formerly called Microccus hyieus

B) •BASED ON THESE TESTS AND RESULTS:

EMB TEST	ACID & FERMENTATION WEAK
PEA	G+/ STAPHYLOCOCCUS
CATALASE	( +) HAVING CATALYST ENZYME
OXIDASE	(-)NO HAVING CYTOCHROME C OXIDASE
PHENOL RED	ACIDIC/ NO GAS PRODUCED
BREWER JAR	AEROBIC
MR/VP TEST	(-)(-) NO FERMENTATION /YELLOW COLLOR
NITRATE TEST	PARTIAL RED (+)
M.I.O	YELLOW - MOTILITY& ORNIHITIN ARE (-)
PEPTON IRON AGAR	(+) H2S PRODUCED

PHENYLALANINE TEST	YELLO COLOR(-)
STARCH	(-) NO PRODUCE AMYLASE ENZYME
SIMMONS CITARATE AGAR	(-) GREEN COLOR
UREASE TEST	YELLOW/ DON'T PRODUCE UREASE ENZYME
GELATINE TEST	LIQUID (+)
MANNITOL SALT AGAR	(+)/ SALT TOLERANT & A LITTLE FERMENTATION
MACcONKEY AGAR	G+/ NO GROWTH
BLOOD AGAR	BETA
KIRBY BAUER	SENSITIVE TO PENICILLIN (P10)

Answer 1

The above mentioned characterstics are of gram positive Staphylococcus hyicus. They are medium sized (0.6- 2 micrometers), white and opaque colonies, cocci shaped. The white and glistening colonies are 1-7 mm in diameter that grows at an optimum temperature of 30-35 C.

They have catalase enzyme, have enzymes for nitrate reduction (nitrate test), acid producing, can grow in Blood agar and mannitol salt agar as these provide nutrients for the culture to grow.