Question

Homework # 2

Answer the following questions based on the given links about the CRISPR- Cas 9 gene editing system:
https://www.nature.com/news/five-big-mysteries-about-crispr-s-origins-1.21294
https://www.youtube.com/watch?v=2pp17E4E-O8

1. Describe F. Mojica’s contribution to the study of CRISPR.
2. How does the CRISPR- Cas9 system work and why is it considered a part of bacterial adaptive immunity?
3. Name 2 applications of the CRISPR- Cas9 system.

Answer 1

1. F.mojika is a honorable microbiologist and have a lot of research in microbiology. He played a important role to effect describe the antiviral defence system and open a gate for recombinant research to develop a useful techniques.

2. CRISPR is reffered as clustered regularly interspaied short palindromic repeats.crispr / cas 9 is a RNA mediated nuclease system that used by bacteria to protect themselves against viral attacks. The whole system relies on RNA and that rna guide for nuclease that break down of nucleotide so probably of a DNA .

When RNA matched with DNA template and then binds together , these nuclease recognise RNA DNA duplex and degrade DNA selectively not the RNA . Cas9 is a nuclease protein.

3. Used in drug development , in agriculture to make a transgenic plant.

Used in fertilized eggs to create a transgenic animals for a targeted mutations.