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Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes...

Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. What are some factors that influences transformation efficiency?

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Answer #1

Factors that effect transformation efficiency are:

Bacterial strains: Gram positive bacteria produce a competency factor which makes their neighbours more competent. This results in increasng the transformation efficiency of the entire colony.

Growth phase: Bacteria can be transformed easily during the log or exponential phase. Tranformation efficiency of bacteria is high when it is rapidly dividing.

State of DNA: Supercoiled DNA has a better transformation efficiency than linearized or straight DNA.

Genotype of cells: Tranformation efficiency is greater for cloning strains due to the presence of mutations.

Methods of transformation: Methods that do not use contaminants have a higher rate of transformation.

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