Question

1. What is the color of bacteria cells in a Negative stain?
2. Unlike the Acid-fast staining, there is no need for a washing step with Negative stain – Why?
3. What bacteria structure can be observed with a Negative stain?
4. What 2 genera contain species that are acid-fast organisms?
5. What makes a microbe an acid-fast organism

Answer 1

when Safranin counterstain allows dye adherence to gram-negative cells,Gram-negative cells appear pink or red

In contrast to direct stains that bind to bacteria directly, a negative stain colors the background of a smear rather than the bacteria.hence no need

Negative stains can demonstrate capsules.

The genus Mycobacterium and the genus Nocardia are among the few bacteria possessing an acid-fast cell wall.

Acid-fast bacteria, also known as acid-fast bacilli or simply AFB, is a group of bacteria sharing the characteristic of acid fastness. Acid fastness is a physical property that gives a bacterium the ability to resist decolorization by acids
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