Question

Describe how RNAi works, and explain how RNAi is involved in post transcriptional regulation.

Describe how RNAi works, and explain how RNAi is involved in post transcriptional regulation.

Homework Answers

Answer #1

RNAi = RNA interference
It is a protective mechanism found exclusively in eukaryotes.
It provides protection against invading RNA genome-containing viruses.

In eukaryotes, the genome is double stranded RNA and it is located in the nucleus.
The RNA is generally dingle-stranded and is found in the cytoplasm.
If there is a double stranded RNA found in the cytoplasm, it indicates an infection.
The recognition of a dsRNA triggers the activation of the RISC compelx.
RISC = RNA-induced silencing compelx
The activated RISC compelx binds to the dsRNA and mediates its cleavage.
It is a post-transcriptional regulatory mechanism i.e. it regulates gene expression after the transcription event.

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