Question

carol s smart wants to determine if her favourite gene is expressed in liver tissue. she performs a northern blot probing with an olgionucleotide complementary to a section of her favourite gene. she sees two bands. she repeats the experiment incubating rhe probe at a temperature of 5 degrees higher. this time only one band appears. which is the best explanation for the results?

a) the band appearing at higher temp is an alternatively spliced form of her favourite gene

b) band appearing st lower temp has a sequence with little similarity to her favourite gene

c) band appearing at low temp has a sequence that is similar but NOT identical to her favourite gene

d) band appearing at low temp is an alternatively spliced form of her favourite gene

e) band appearing at high temp has a sequence that is distantly related to her favourite gene

Answer 1

Answer :- Band appearance at high temperature has a sequence that is distantly related to her favourite gene as the 2 bands that appears in the first case is the gene that is almost similar to the favourite gene and the second band is of the favourite gene that is expressed in the liver . At higj temperature the band of the favourite gene disappear due to the distoration of the oligonucleotide sequences as in the case of northern blotting . On the increase in temperature , only those gene expressed that are the stress responsible gene . These genes are also responsible to the oxidative stress and leads to their degradation

Hence option E is the correct answer

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