Question

What are the limitations of the Biuret test for proteins?

What are the limitations of the Biuret test for proteins?

Homework Answers

Answer #1

biuret test is coducted to detect existence of peptide bonds in proteins. peptide bonds turns the sample to purple colour on reacting with copper ions. the protein proportion will be identified based on the intensity of the purple colour.
the limitations include,
1. it indicates only presence of peptide bonds but not the actual count of the bonds in the protein.
2. colour of the other componenets in the sample can fluctuate the purple colour of the protein.
3.this test will not detect amino acids in the protein.
4. this test will not detect the very small peptide bond concentrations in the proteins.
5. this test will not give accurate results if the sample is not diluted properly or if it is blurred and below the standard value of 5mg/ml

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
what are the limitations in a confirmatory test in molecular diagnostics to diagnose a disease?
what are the limitations in a confirmatory test in molecular diagnostics to diagnose a disease?
What are 3 limitations of ARR? what are 3 limitations of PBP?
What are 3 limitations of ARR? what are 3 limitations of PBP?
Exercise Prescription Questions: 1.) What are some limitations associated with the Rockport fitness walking test? 2.)...
Exercise Prescription Questions: 1.) What are some limitations associated with the Rockport fitness walking test? 2.) What are some issues related to HR palpation? Are there any procedural concerns? If so, what are they?
What are the limitations of trend line long-term extrapolation? What are the limitations of moving average...
What are the limitations of trend line long-term extrapolation? What are the limitations of moving average technique? NOTE: Your answer to each part must include at least two references. Give page numbers where applicable.
What are the limitations of ecological pyramids?
What are the limitations of ecological pyramids?
What are the limitations of intersection testing?
What are the limitations of intersection testing?
What are the limitations of ecological pyramids?
What are the limitations of ecological pyramids?
What are the limitations of ecological pyramids?
What are the limitations of ecological pyramids?
what are some of the limitations of regression analysis?
what are some of the limitations of regression analysis?
What are the advantages and limitations of simulation models?
What are the advantages and limitations of simulation models?
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT