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If you attached the gene for insulin to the gene for PDI and then express this...

If you attached the gene for insulin to the gene for PDI and then express this recombinant protein in a cell, where would you expect the insulin to end up and why? If there was no COP1 available would this have an impact on where in the cell the recombinant insulin ended up?

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Answer #1

PDI or protein dulsfide isomerase is an enzyme which is expressed in endoplasmic reticulum. This enzyme is responsible for the proper folding of proteins. PDI performs this function as they can break the disulfide bond which is present in between cystine residues of protein bond. Thus when recombinant insulin gene is attached to PDI it will directed towards endoplasmic reticulum.

on the other hand COP1 vesicles are pat of the retrograde transport between golgi complex and endoplasmic reticulum. They coat and helps protein to transport from the cis end of the golgi complex to rough endoplasmic . reticulum. proteins which are secreted transported back to endoplasmic reticulum. Thus if no COP1 is available recombinant insulin will ended up in golgi.

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