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1.Optimal Salt Concentration, are Bacillus subtilis halophile or halotolerant, or neither? 2.What media best supports the...

1.Optimal Salt Concentration, are Bacillus subtilis halophile or halotolerant, or neither?

2.What media best supports the growth of Bacillus subtilis?

please provide scholarly source for answers

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Answer #1

1. Bacillus subtilis is halotolerant Halotolerant bacterial is able to grow at higher salt concentration which is normally required for their growth.

Purification and characterization of a halotolerant intracellular protease from Bacillus subtilis strain FP?133. Journal of basic microbiology, 2006.

Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin.Extremophiles. 2002 Dec;6(6):499-506.

2.Plate Count Agar (PCA) medium.

Bacillus Classification Based on Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry—Effects of Culture Conditions, Scientific reports, 2017

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