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would you use metatranscriptomics, metagenomics, or analyzation of 16s rRNA genes to observe the changes in...

would you use metatranscriptomics, metagenomics, or analyzation of 16s rRNA genes to observe the changes in cyanobacterial growth day vs night. explain why and what you would find from the method.
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One shall use metatranscriptomics in order to study the changes in cynobacterial growth during day vs during night. Tranacript level vary according to the requirement of their corresponding proteins. Hence studying the varkari on in transcripts would give a preliminary lead towards the mechanism of growth during night and day.

Metagenomics is the study of genetic material, here the DNA, obtained from an environmental source. Genome of and organism shall never change in an organism, at the sequence level

16s rRNA remains majorly among the prokaryotic organisms and hence will not changes over day and night. It codes for a ribosomal protein which is essential and hence constitutively expressed.

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