Question

. Suppose you start with 1 molecule of glucose and go through glycolysis to produce 2 molecules of pyruvate. These 2 molecules of pyruvate are then converted into acetyl CoA which then go through the TCA cycle. Approximately how many total ATP / ATP-equivalents can be formed through this process? (Account for NADH/FADH2 produced). Show where each molecule of ATP is generated from.

Answer 1

#Energy gain (ATP)/glucose in glycolysis under aerobic condition:

Rea:1(hexokinase)-1 ATP

Rea 3(PFK)-1 ATP

Rea 6(G 3-PDH)-2.5*2=5

Rea 7(BPGK)+1*2=2

Rea 10(PK)+1*2=2

Total=9-2=7 ATP

#Energy gained in TCA

isocitrate DH 2.5*2=5 ATP

ketoglutarate DH 2.5*2=5 ATP

S.thiokinase 1*2=2 ATP

S. DH 1.5*2=3 ATP

Malate DH 2.5*2=5 ATP

Total=20 ATP

#Net ATP generation in glycolysis 9-2=7 ATP

ATP generation in PDH 2.5*2=5 ATP

ATP generation in citric acid cycle 20 ATP

therefore net generation of ATP from one glucose molecule= 32 ATP