A nanodrop cannot tell RNA for a DNA sample. How do you make
sure RNA quality is acceptable? How do you determine the
The quantity and quality of RNA can be determined by the UV absorption by means of a UV spectrophotometer.
The maximum absorption of RNA occurs at 260 nm, so its concentration can be determined by its optical density at 260 nm. A260 of 1.0 is equal to 40 µg/mL RNA.
The protein contamination (RNA purity) can be determined by calculating the A260/A280 ratio. The A260/A280 ratio of a pure RNA is, 2.1; and the purity is acceptable if the ratio ranges between1.8 to 2.0.
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