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Question: The P5 adaptor is important to next generation sequencing because it: Options: 1) This is...

Question: The P5 adaptor is important to next generation sequencing because it:

Options:

1) This is the adaptor that binds to the DNA sequence

2) This is the adaptor that binds to the primer

3) This is the adaptor that indexes the samples

4) This is not used in next generation sequencing
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Answer #1

Option - 3 is the correct answer. The P5 adaptor is important to next-generation sequencing because it has a unique property to indexes the sample. With this method, multiple sequences can be sequenced together. They form correct base pairing by reducing the errors to a minute level and also maintain a balance between the base pairs.

Other options like the binding of an adaptor to the DNA sequence and primer are incorrect as they are designed for specific sequences not for the whole DNA sequence or a primer. So it is incorrect. The statement that it is not used in next-generation sequencing is also incorrect as this a very technique and plays a major role in advanced generation sequencing. So, it is also incorrect,

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