Select a single most appropriate statement in the following questions. Each question is worth two marks.
Q1 Transcription from a mutated gene gets aborted only after synthesis of 10 or 15 bases long mRNAs. If the sequence of the 10 base mRNA is 3`-AUGCUAGCGG-5`; the sequence of 15 base mRNA transcribed by this mutated gene will be
a. 5`-GGCGAUCGUA AUGGG-3`
b. 5`-AUGGG GGCGAUCGUA -3`
c. 5`-AUGCUAGCGG AUGGG-3`
d. 5`-AUGGG AUGCUAGCGG -3`
e. 5`-UACGAUCGCC AUGGG-3`
Q2 In transcription, the Rho protein is involved in
a. Initiation
b. Elongation c. Termination d. Open complex formation e. Poly A tail synthesis
Question 15) option A is correct
5`-GGCGAUCGUA AUGGG-3`
As we know that any synthesis process occurs in 5' to 3' direction.
So here as told in question that mutated gene form 10 or 15 base pair long mRNA.
So both 10 or 15 base will have same 5' end. So here in the option only a option has similar to 5' of the 10bp mRNA so this is the correct answer.
Question 2) option c, termination
In prokaryotes two types of termination occur
Rho independent- here mRNA form stem loop like structure and do not require any protein
Rho dependent- here rho protein involve in termination.
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