Question

Microbiology, i am a bit confused right now. i have been given an assigment to identify a bacteria i am workring with and answer some questions using scholarly sources. the only issue is i am finding it hard to firgure out which source to use as they contradict each other sometimes.

The Bacteria is Bacillus Subtilis(well known)

I have the following questions, although i have answered them all; i still need clarification and another source. please attach a link to all the sources used so i can take a look at them. thanks

Question	My answers
Glucose Fermentation Phenotypes (Growth? With Acid? With Gas)	grows sometimes, (idk the rest cant find sources)
Term to describe metabolism: (chemoheterotroph, photoheterotroph, chemoautotroph, chemolithotroph, photoautotroph)	chemoheterotropic (im sure of this but some sources have stated otherwise)
Media that best supports the growth of this organism: Bacilus Subtilus	rich media and selective sounds most likely (some sources have said glucose and minimal for some reason)
Temperature requirements for optimal growth: Term to describe this organism with respect to temperature requirements:	37 degrees (but some have say lower at 30 degrees)
Optimal Salt Concentration. Is the organism a halophile or halotolerant, or neither?	halotolerant
Lactose Fermentation Phenotypes (Growth? With Acid? With Gas)	usually does not ferment lactose. (idk the rest cant find sources)

Answer 1

Source of glucose fermentation: streptococcus bovis- Gram positive bacteria in humans associated with urinary tract infections, Enterobacter aerogenes, Serratia marcescens, Shigella flexneri, Citrobacter frundii.

Photoheterotrophic- Oriental hornet

Chemoautotrophic- Nitrosomonas, methanogens, iron bacteria

Chemoautotrophic- Thiobacillus ferroxidans

Photoautotrophs- pleurocapsales, RhodobacterMedium for B. subtilis- peptone yeast extract

medium, nutrient agar medium, Difco sporulation medium, Luria bertani broth

Optimal temperature is 30- 40 °c.

Lactose fermentation phenotypes- klebsiella pneumoniae, Escherichia Coli, Serratia, Enterobacteriaceae.