Question

Question 1:

A. Explain the chemical differences between DNA and RNA and their biologic significance.

B. Explain how to identify the chemical structure of each nucleoside base present in DNA or RNA.

C. Explain the characteristics of DNA polymerase III (replication rate, error rate, etc).

D. Explain the characteristics of RNA polymerase (replication rate, error rate, etc.).

Answer 1

A. the difference between is (a) DNA contain deoxy ribose nucleic acid and different sugar that lack one oxygen atom and (b) RNA contain ribose nucleic acid that nucleobase uracil and contain thymine.

B. they are contain only four different nuceotides the adenine, guinine, and cytosine are found both in DNA and RNA. the chemical structure of the principal bases in nucleic acid. according heterocuclic bases nuccleosides can be isolated from nucleic acid and to establish the structure of each nucleosides present in the nucleid acid.

C. they are involve in replication activity. The DNA pol3 holoenzyme also proofreading activity reading 3'-5' and synthesizing 5'-3' DNA Pol 3 is component pf replisome.

D. they consist four major subunit beta', beta, alpha, gama with molecular weight of 160,150, 86, 40 kdaa rifampin, sulfydryl they are inhiobitor of E. coli RNA polymerase.