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The PCR products are a certain length due to: A) The DNA breaking off B) Restriction...

The PCR products are a certain length due to:

A) The DNA breaking off

B) Restriction enzymes

C) The heat in the PCR machine

D) The placement of the primers

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Answer #1

D) The placement of the primers.

Primer is responsible for binding to the desired template of DNA and start replication from there. It is non-specific and can bind to any region of the template hence leading to DNA products of different sizes. If there is a repeat in the gene sequence, the primer would allow its replication also leading to the different sized product. Restriction enzymes are responsible for identification and isolation of gene of interest from a large DNA fragment such as plasmids. DNA breaks off during termination and the heat is responsible for denaturation of DNA.

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