Question

Which are components of a PCR reaction? Check All That Apply RNA primers Dideoxyribonucleotides DNA ligase...

Which are components of a PCR reaction?

Check All That Apply

  • RNA primers

  • Dideoxyribonucleotides

  • DNA ligase

  • Heat–stable DNA polymerase

  • Template DNA

  • Deoxyribonucleotides

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Heat–stable DNA polymerase

Template DNA

Deoxyribonucleotides

Components of PCR -

Component Role
Primer pair binds to 3' end of template DNA to provide free OH group to DNA polymerase
Template DNA DNA molecule containing gene of interest
Taq polymerase heat stable DNA polymerase which adds dNTPs to the growing DNA molecule
MgCl2 provides magnesium ions which chelates and inactivates DNases
dNTPs monomers of DNA
Buffer to maintain a constant pH
Water to make up the final volume

Please rate high.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
What occurs during the annealing stage of a PCR reaction? A. The reaction stops B. The...
What occurs during the annealing stage of a PCR reaction? A. The reaction stops B. The DNA polymerase copies the template DNA C. The primers bind to the template DNA. D. The DNA strands separate The PCR protocol that is currently used in laboratory was facilitated by a discovery of bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat stable form of DNA Polymerase known as Taq polymerase, which continues...
As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is...
As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is based on the way DNA is replicated in cells. During PCR template DNA plus other ingredients are placed in a test tube and the solution goes through repeated cycles of heating and cooling. You can find more information about PCR here. The 'ingredients' of a basic PCR reaction are: DNA template, primer, nucleotides, DNA polymerase, and any salts required for polymerase function (buffer). No...
During the PCR reaction, the DNA sample is heated in order to separate it into single...
During the PCR reaction, the DNA sample is heated in order to separate it into single strands. allow primers to bind. synthesize RNA. allow DNA polymerase to work.
1. This enzyme replaces RNA primers with a compatible DNA segment. RNA Primase DNA Polymerase III...
1. This enzyme replaces RNA primers with a compatible DNA segment. RNA Primase DNA Polymerase III Topoisomerase DNA Polymerase I 2. This class of enzymes prevent DNA molecules from damaging themselves by relieving strain, untangling, cutting and rejoining strands. They will ride ahead of the replication fork preventing over-winding. Helicase DNA Ligase Topoisomerase RNA polymerase 3. This type of molecule prevents the two separated strands of DNA in a replication bubble from re-establishing their hydrogen bonds ("reannealing"). DNA Polymerase III...
A master mix contains a mixture of all the reagents needed for a PCR. What are...
A master mix contains a mixture of all the reagents needed for a PCR. What are the reagents? A) DNA, primers, and nucleotides B) Primers, nucleotides, and polymerase C) DNA, RNA, and nucleotides D) RNA, nucleotides, and polymerase
what are Polymerase chain reaction requirements, (template DNA, dNTPs, primers)
what are Polymerase chain reaction requirements, (template DNA, dNTPs, primers)
The prokaryotic replication enzyme that removes and replaces primers is _______. a. ligase b. DNA polymerase...
The prokaryotic replication enzyme that removes and replaces primers is _______. a. ligase b. DNA polymerase I c. primase d. DNA polymerase II Primers are found on: a. only Okazaki fragments. b. both the leading and lagging strands. c. only the leading strand. d. only the lagging strand. e. DNA polymerase III. Which replication enzyme seals the nicks between Okazaki fragments? a. Primase b. DNA polymerase I c. DNA LIgase d. Topoisomerase The first step in the replication of DNA...
You have access to a PCR machine and all the reagents, (primers, Taq polymerase etc). You...
You have access to a PCR machine and all the reagents, (primers, Taq polymerase etc). You do the PCR reaction on your unknown DNA and you sequenced your PCR product. The sequence you obtain is the following: AGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGA CTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTT GAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCAC CTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTC What is your unknown?    How did you come to that conclusion?
Which of the following statements about primers are correct? Select all that apply. A. They are...
Which of the following statements about primers are correct? Select all that apply. A. They are synthesized by an enzyme called primase B. They provide a 3-OH group for atachment of DNA nucleotides. C. They are needed for the start of DNA synthesis. D. They are synthesized by DNA polymerase α.
Which of the following statements about primers are correct? Select all that apply. A. They are...
Which of the following statements about primers are correct? Select all that apply. A. They are synthesized by an enzyme called primase. B. They provide a 3-OH group for attachment of DNA nucleotides. C. They are needed for the start of DNA synthesis. D. They are synthesized by DNA polymerase α.
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT