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Which are components of a PCR reaction? Check All That Apply RNA primers Dideoxyribonucleotides DNA ligase...

Which are components of a PCR reaction?

Check All That Apply

  • RNA primers

  • Dideoxyribonucleotides

  • DNA ligase

  • Heat–stable DNA polymerase

  • Template DNA

  • Deoxyribonucleotides

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Homework Answers

Answer #1

Heat–stable DNA polymerase

Template DNA

Deoxyribonucleotides

Components of PCR -

Component Role
Primer pair binds to 3' end of template DNA to provide free OH group to DNA polymerase
Template DNA DNA molecule containing gene of interest
Taq polymerase heat stable DNA polymerase which adds dNTPs to the growing DNA molecule
MgCl2 provides magnesium ions which chelates and inactivates DNases
dNTPs monomers of DNA
Buffer to maintain a constant pH
Water to make up the final volume

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