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What are the potential sources of error in Bradford’s assay and LDH activity test?

What are the potential sources of error in Bradford’s assay and LDH activity test?

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Answer #1

Sources of error in Bradford assay

The absorbance of the protein sample is lower than expected

The absorbance of the protein sample is higher than expected

The absorbance of the standard is lower than expected

Samples are dark blue

Detergents in your protein buffer:

Dialyze your protein sample or dilute the sample to reduce the level of detergents.

Sources of error in LDH activity

Hemolysis RBCs found in serum(contain 100-150 times )

Analyte stability

Run assay asap or store at room temperature

Prolonged contact of serum and cells

use of poor quality of reagents e.g NADH & NAD+, homeolysed sample

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