Question

If you wished to design a molecular approach that would distinguish between live and dead bacteria,...

If you wished to design a molecular approach that would distinguish between live and dead bacteria, what would you suggest doing?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

When a bacteria is given a nutritional stimulus, the bacteria that is alive will produce a specific rRNA molecule in response to that stimulus but a dead bacteria will not produce it. Hence in live bacteria the levels of rRNA gradually increases whereas in dead bacteria the rRNA levels wont increase at all. This can be identified by designing specific primers for that rRNA and performing a reverse transcriptase-qPCR (RT-qPCR) where we can identify the levels of rRNA gradually increasing in live cells and in dead cells this levels will be constant. This is one of the molecular approach to distinguish between live and dead bacteria.

If you have any query kindly comment before giving thumbs up. Thank you.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
If you wished to design a molecular approach that would distinguish between live and dead bacteria,...
If you wished to design a molecular approach that would distinguish between live and dead bacteria, what would you suggest doing?
Distinguish between F+ and F- bacteria. What is the difference between F+ and Hfr bacteria?
Distinguish between F+ and F- bacteria. What is the difference between F+ and Hfr bacteria?
Halophilic bacteria have been developed to live in a very salty environment, e.g. Dead Sea. The...
Halophilic bacteria have been developed to live in a very salty environment, e.g. Dead Sea. The most important salts in the Dead Sea are MgCl2 (1.83 M), NaCl (1.78 M), CaCl2 (0.44 M) and KCl (0.20 M). Typical for halophilic organisms is that their proteins have a much larger (negative) net charge than proteins in organisms that live at normal salinity. (a) Explain why the proteins of halophilic organisms need a large net charge in order for the proteins not...
Which of the tests discussed in this experiment you would use to distinguish between the following...
Which of the tests discussed in this experiment you would use to distinguish between the following pairs of compounds. Describe, in one sentence exactly what you would observe doing that test. 1. 2-bromobutane and 2-chlorobutane 2. Isopropyl alcohol and n-propanol 3. 1-butanol and 1-octanol 4. Butyl amine and butanic acid
Suggest a chemical test that would allow you to distinguish between tert-butyl alcohol and 1-butanol, both...
Suggest a chemical test that would allow you to distinguish between tert-butyl alcohol and 1-butanol, both of which give a positive ceric nitrate test.
Construct a recombinant bacteria strain with a gene mutated. You need to design and suggest the...
Construct a recombinant bacteria strain with a gene mutated. You need to design and suggest the steps. 1) cloning of gene 2) mutation (how to do it) 3) which homologous recombinant method you use 4) how to verify the strain Explain the steps in detail.
What is the fundamental distinction between design capacity and effective capacity? Provide a brief example. Distinguish...
What is the fundamental distinction between design capacity and effective capacity? Provide a brief example. Distinguish between utilization and efficiency. Why is the capacity decision important?
How would you distinguish between the following pairs by use of infrared Spectroscopy only? Tell what...
How would you distinguish between the following pairs by use of infrared Spectroscopy only? Tell what absorption would be present or absent in each case. A. 2-pentanone and 2-pentanol B. Acetophenone and p-tolualdehyde C. 3-pentanone and benzophenone How would you distinguish between the pairs a bowl of using only proton NMR spectroscopy? Sketch a possible spectrum for each
Bacteria that live in extreme temperatures (either very hot or very cold) have substantially different fatty...
Bacteria that live in extreme temperatures (either very hot or very cold) have substantially different fatty acids in their phospholipids. How would these fatty acids differ between bacteria living in hot and cold extremes? Why? What other molecules might differ in the membranes of bacteria living at these extreme temperatures. Why?
How would you distinguish between the IR spectrums of Benzil, Bibenzyl, and Biphenyl? Identidy what major...
How would you distinguish between the IR spectrums of Benzil, Bibenzyl, and Biphenyl? Identidy what major peaks would be present and what functional groups they would correspond to.
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT