Question

4.      (1 point) The enzyme ?-galactosidasecan hydrolyze the disaccharide allolactose into glucose and galactose. If you want...

4.      (1 point) The enzyme ?-galactosidasecan hydrolyze the disaccharide allolactose into glucose and galactose. If you want to monitor the progress of allolactose’s hydrolysis reaction, which assay will work the best?  Explain your reasoning.

Benedict's test

Barfoed's test

Seliwanoff's test

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Barfoed's test :

Barfoed's test is a chemical test used for detecting the presence of monosaccharides.

It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu2O), which forms a brick-red precipitate.

RCHO + 2Cu2+ + 2H2O ? RCOOH + Cu2O + 4H+

The aldehyde group of the monosaccharide which normally forms a cyclic hemiacetal is oxidized to the carboxylate. A number of other substances, including sodium chloride, may interfere.

It was invented by Danish chemist Christen Thomsen Barfoed and is primarily used in botany.

Benedict's test is use for reducing sugara.

Seliwanoff's test is use for aldose and ketose sugars.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
1.   The disaccharide groups are: * 1 point A. Fructose, maltose, Galactose B. Lactose, fructose, glucose...
1.   The disaccharide groups are: * 1 point A. Fructose, maltose, Galactose B. Lactose, fructose, glucose C. Sucrose, glucose, Galactose D. Sucrose, lactose, maltose 2.   Which of the following are NOT used as fuel and are required in small quantities? * 1 point A. Essential nutrients B. Lipids C. Macronutrients D. Micronutrients 3.   Which of the following stores the greatest amount of energy in the body? * 1 point A. Vitamins B. Proteins C. Glycogen D. Minerals 4.   Proteins serve...
Sucrose, a disaccharide, is often added as a sweetener, but it is not as sweet as...
Sucrose, a disaccharide, is often added as a sweetener, but it is not as sweet as its constituent monosaccharides, D-glucose and D-fructose. Besides enhancing sweetness, fructose has hygroscopic properties that improve the texture of foods, reducing crystallization and increasing moisture. In the food industry, hydrolyzed sucrose is called invert sugar, and the yeast enzyme that hydrolyzes it is called invertase. The hydrolysis reaction is generally monitored by measuring the specific rotation of the solution, which is positive ( 66.4°) for...
what would be the best buffering agent to choose if you want to buffer an enzyme...
what would be the best buffering agent to choose if you want to buffer an enzyme reaction or tissue culture at 7.2    a. hepes (4-(2-hydroxyethyl)-1piperazineethanesulfonic acid) pk =7.5 b. histidine pk 1=1.78; pk2=5.97; pk3=8.97 c. bicarbonate; pk=6.1 d. phosphate; pk1=2.0, pk2=6.8; pk3=12.5   e. tris or tris(hydroxymethyl)aminomethane; pk=8.07
1) Catalase Enzyme Activity. After heating the food item for 5 minutes the reaction rate of...
1) Catalase Enzyme Activity. After heating the food item for 5 minutes the reaction rate of catalase increased the hot food item caused the hydrogen peroxide to break down into water and O2 the high heat caused the catalase reaction not to occur none of the above 2)Hydrolysis of starch. You were asked to let the starch solution cool. Once the solution had cooled and was back to room temperature you would describe your glass as all the starch dissolved...
1- Imagine that you want to build a radio telescope and an optical telescope and that...
1- Imagine that you want to build a radio telescope and an optical telescope and that you want them to both have the same angular resolution, assume that both telescopes can reach the diffraction limit. Which telescope will have to have a larger diameter? Explain your reasoning. 2- How many times larger is the collecting area of a 4-meter telescope than a 1-meter telescope?
1. If you doubled the amount of charge on each electrode for the point-point configuration, what...
1. If you doubled the amount of charge on each electrode for the point-point configuration, what changes would take place in the electric field lines? What changes would take place in the equipotential lines? Explain the reasoning behind your answers. 2. Imagine an electron in the conducting paper that moves on a straight line from the negative to the positive electrode. Compare the work done on the electron for each set of electrodes you used during Part One of the...
BIOCHEMISTRY ASSIGNMENT Please use your understanding to address the few concerns below * Required Index Number:...
BIOCHEMISTRY ASSIGNMENT Please use your understanding to address the few concerns below * Required Index Number: * Your answer Program: Choose BSc Midwifery BSc Nursing Which of the diagrams illustrates the way in which the rate of an enzyme-controlled reaction depends on substrate concentration? * Captionless Image A B C D When starch and amylase are mixed in the presence of iodine solution, the blue-black colour rapidly disappears. This is due to the formation of which molecule? * glucose glycogen...
You isolate an enzyme from the human stomach to test its activity. In order to measure...
You isolate an enzyme from the human stomach to test its activity. In order to measure optimal enzyme function, which of the following laboratory conditions should you use in the experimental reactions? a. Solutions with a pH ~2 b. Reaction temperature close to normal body temperature (98.6°F) c. Include substrates for which the enzyme has high affinity d. Include high concentrations of substrate e. All of the above In glycolysis, glucose-6-phosphate (G-6-P) is converted to fructose-6-phosphate (F-6-P). Which of the...
1.  You want to retire and have annual payments of $55,000 over a 23 year period.  You plan...
1.  You want to retire and have annual payments of $55,000 over a 23 year period.  You plan to retire in 16 years.  If you can earn 7.5% on your funds, how much do you need to invest monthly until you retire to reach your goal? 2.  You have a choice of $1 million in 50 years or $1,600 today.  If your interest rate is 14%, which would you choose?  (show your work to receive credit). 3.  You are looking at 2 investments.  One will pay you $550...
Activity 1: Scientific Reports You may have heard the question “If a tree falls in a...
Activity 1: Scientific Reports You may have heard the question “If a tree falls in a forest and no one is around to hear it, does it make a sound?” A similar question can be asked about experiments. “If a researcher performs an experiment and never publishes the result has science been performed?” Many people would say no because science is the accumulation of knowledge. If the results of an experiment are not published, knowledge is not gained. The final...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT