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How would you decide what percentage of agarose to use to resolve the DNA fragments? How...

How would you decide what percentage of agarose to use to resolve the DNA fragments? How would you select an appropriate molecular weight marker to compare the fragments to?

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Answer #1

The greater the percentage of agarose, the smaller the linear DNA that can be resolved.

The percent of agarose needed is strictly based on some experimental results, so using the previously conducted experiments we can roughly estimate the concentration of agarose needed.

I recently came across some data which might be helpful to you. Molecular weigh marker for DNA are of two types Standard and digested. Both got their names from the processes they are formed. Standard is the most famous one as it is useful for the most medium sized DNA and vector fragments. Both types of markers are commercially available.

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