Question

What are the advantages and disadvantages of using EcoRI vs BamHI as the restriction enzyme for the digest? Thank and happy easter!

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Answer 1

Advantages:

these enzymes are rapidly available in Laboratories or production companies.

due to high availability most of these enzymes is less as compared to other enzymes.

the sequence this enzymes have more chances to occur in DNA.

these enzymes are less reactivewith other components.

Disadvantage:

Both recognizes 6 base pair palindromic sequences, so the chances of occur in the sequence is one after 4096 nucleotides.

we can say that they have availability of sequences but the chances in the short DNA is very.

the availability for the sequences are only for long DNA.

they both produce stickiness and demand of blunt end cannot be fulfilled by these enzymes.