Question

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1) How can we measure relative mRNA amount using RT-PCR?

Answer 1

Reverse transcription (RT) trailed by polymerase chain reaction (PCR) is at exhibit the strategy for the identification of particular RNA molecule. Evaluation of nucleic acids utilizing the PCR has been essentially rearranged by the improvement of the constant PCR strategy, where the fluorescent dye in the PCR mixture is distinguished in each intensification cycle. In biological applications looking at gene expression, Relative mRNA evaluation is an approach deciding the quantity of target mRNA in tests relative each to other. To adjust for contrasts in the RT-PCR input quality and amount, the target mRNA sum in each biological sample is standardized to at least one internal controls.