Question

2. What does the term “precipitation” mean, in the context of this lab? For example, we will precipitate the DNA out of solution using alcohol.

3. What is the meat tenderizer used for?

4. In a DNA double helix an adenine of one strand always pairs with a(n) _____ of the complementary strand, and a guanine of one strand always pairs with a(n) _____ of the complementary strand.

5. An old (parental) DNA strand is used as a _____ for the assembly of a new DNA strand.

Answer 1

2.Precipitation :

Precipitation refers to aggregation of insoluble particles of a liquid mixture by centrifugation.

DANA is insoluble in ethanol (ice cold).It aggregates together giving a pellet upon centrifugation.

It is useful in DNA precipitation assay.

3.Meat tenderizer :

It acts as protease enzyme (proteolytic).It breaks down peptide bonds of proteins into amio acids.

In case of DNA extraction, Meat tenderizer cuts the proteins away from DNA.(In the nucleus, the DNA is molded, folded and protected by proteins). Examples of meat tenderizer are bromelain, papain.

4.Thymine ,. Cytosine

5.template