Question

On occasion, DNA polymerase will incorporate a wrong nucleotide in the new DNA strand, resulting in...

On occasion, DNA polymerase will incorporate a wrong nucleotide in the new DNA strand, resulting in a mismatch pair. Which of the following is the more commonly produced mismatch?

Group of answer choices

AT

GT

GC

CC

When a new strand of DNA is being synthesized,

Group of answer choices

An ionic bond at the 3’ position of the existing strand and the 5’ position of the new nucleotide is generated

A phosphodiester bond at the 3’ position of the new nucleotide and the 5’ position of the existing strand is generated

A phosphodiester bond at the 5’ position of the new nucleotide and the 3’ position of the existing strand is generated

An ionic bond at the 1’ position of the new nucleotide and the 3’ position of the existing strand is generated

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Answer #1

Most commonly produced mismatch in DNA is GT.

DNA is double helix nucleotide structure. It contains 4 nitrogen bases, Adenine, Guanine, Cytosine and Thymine. Normally in DNA, Adenine(A) form hydrogen bond with Thymine (T) and Guanine (G) form hydrogen bond with Cytosine (C).They are known as base pair.Most common mismatch in base pairing by DNA polymerase is G-T.

When a new strand of DNA is being synthesized -

A phosphodiester bond at the 5’ position of the new nucleotide and the 3’ position of the existing strand is generated.

DNA polymerase is an enzyme which elongates the DNA strand during replication. But DNA polymerase can add nucleotide only to the 3' OH end of the existing strand. The phosphodiester bond forms between 3' end of existing strand and 5' end of the coming nucleotide. So the synthesis of DNA occurs in 5' -> 3' direction.

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