Question

Make an algorithm to identify the following species

E.coli
Shigella
Klebsiella
Citrobacter
Serratia
Enterobacter
Yersinia
Salmonella
Providencia
Proteus

based on the following tests

OXIDASE
ONPG
INDOLE
TSI
H2S
CITRATE
GLUCOSE
MOTILITY
ORNITHINE
PAD/PPA
UREASE

Answer 1

Test	Positive control Bacteria	Negative control Bacteria
Oxidase	Pseudomonas aeruginosa	E.coli
ONPG	E.coli	Proteus mirabilis
Indole	E.coli, Proteus, Klebsiella	Proteus mirabilis
TSI	E.coli	Shigella
H2S	Proteus vulgaris	E.coli
Citrate	Serratia, Klebsiella pneumoniae	Shigella, E.coli
Glucose	E.coli, Proteus mirabilis	Pseudomonas aeruginosa
Motility	E.coli	Staphylococcus aureus
Orinithine	Enterobacter	Klebsiella
PAD/PPA	Proteus	E.coli
Urease	Proteus	E.coli