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8. You are preparing a 50mL culture of E. coli in LB broth containing the antibiotic...

8. You are preparing a 50mL culture of E. coli in LB broth containing the antibiotic apramycin. The culture media will also need to include L-arabinose to induce gene expression. Answer the following questions and show all work.

a. How would you make 15mL of a 1M stock of L-Arabinose (MW = 150g/mol)? (1pt)

b. How much L-arabinose (from the stock in 8a) do you add to make the final concentration of L-Arabinose in the culture 10mM (.010M)? (1pt)

c. How would you make 10mL of a 50mg/mL stock of apramycin? (1pt)

d. How much apramycin (from the stock in 8c) do you add to make the final concentration of apramycin in the culture 50μg/mL? (1pt)

e. What is the volume of LB broth required to create this 50mL culture? (1pt)

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