Question

A group of investigators has cloned a gene encoding a mouse enzyme and attempted to express it in yeast. The gene is functional in yeast but expressed at very low levels. How might the investigators alter their clone to achieve higher expression levels in yeast without altering the sequence of the protein produced

Answer 1

Hi,

The investigators can use the promoters to increase the expression of the genes in the Yeast clones. This technique is used in various bioreactors to get a maximum yield in the production of enzymes. The promoters helps to enhance the production of these enzymes by improving the gene expression in the Yeast.

There are two types of promoters Constitutive promoters, which provides a stable expression leves in the medium throughout the course of time and Inducible promoters which increases the gene expression through an environmental stimuli. In this case inducible promoters is the best option.

Some examples of promoters are 3-phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase and alcohol dehydrogenase.

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