Compare how glutamate is formed in plants and bacteria versus its formation in animals
In Bacteria and plants contain NADPH specific enzyme to generate
glutamate formation. Bacteria use Ammonia as
nitrogen source for primary nitrogen assimilation.
?-ketoglutarate + NH4+ + NADPH -----L-glutamate
dehydrogenase -- L-glutamate + NADP+ + H2O
Animal enzymes uses alpha ketoglutarate for citric acid cycle. It uses NAD+ as a principal cofactor.
? ketoglutarate + Glutamine + NADPH + H+ -----Glutamate dehydogenase ------ 2 Glutamate + NADP+
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