Question

When using the enzyme coupled reaction for the glucose assay, it is important that the absorbance value determined @415nm has a direct correlation to the amount of glucose in the solution.

Glucose + O2 + H2O ----->Gluconic Acid + H2O2 H2O2 + ABTS(reduced) [colourless] ------> ABTS+(oxidized) [blue]

After reviewing the glucose oxidase/peroxidase-coupled reaction above discuss the following.....

a.) How would your unknown glucose concentration be affected if by some chance a strong reducing agent was a minor contaminant in the Enzyme Colour Reagent? Explain your reasoning.

b.) How would your unknown glucose concentration be affected if by some chance a strong oxidizing agent was a minor contaminant in the Unknown Glucose Sample? Explain your reasoning.

Answer 1