Question

6. Which of the following can be manipulated to promote amino acid production in microbes?

A. Alteration of promotor to increase binding of the repressor.
B. Decrease in cell membrane permeability to prevent release of the amino acid.
C. Alteration of enzymes in pathways such as the TCA cycle to halt the pathway and “push” the intermediate towards the production of the amino acid.

D. A and C.

8. Which of the following is true about Polymerase Chain Reaction (PCR)?
A. Results in the rapid amplification of a specific fragment of DNA, overriding the necessity of a restriction enzyme (RE).
B. Results in the rapid amplification of a specific fragment of DNA, overriding the necessity of running gel electrophoresis to isolate the piece of DNA treated by REs.
C. A PCR product can be inserted into a vector to create recombinant DNA.
D. All of the above choices are true.

Answer 1

Q6:- Option C

Explanation:- The amino acid production can be promoted in microbes by Alteration of enzymes in pathways such as the TCA cycle to halt the pathway and “push” the intermediate towards the production of the amino acid.Several intermediates of krebs cycle such as alpha ketuglutarate , oxaliacetate etc act as precursors for amino acid synthesis

Q8:- Option D

Explanation:- All of the above choices are true i.e pcr overriding the necessity of restriction enzymes, running gel electrophoresis etc