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Why would a Nucleus stain differently at different times?

Why would a Nucleus stain differently at different times?
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Answer #1

Depending upon which phase of the cell cycle:

Staining agents (such as EtBr) usually intercalate between DNA bases and show up on spectrometers.
As such, the morphology of DNA, whether dispersed as fibres, condensed as chromatin, assuming the X-like chromatid structure, these are all possibilities in distribution of cells that are various stages of the cell cycle.
As such, the nucleus stain appears differently. (Consider the blue coloured genetic material in the diagram above)

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