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You repeat this pcr experiment. After you ran a gel, you realize that you forgot to...

You repeat this pcr experiment. After you ran a gel, you realize that you forgot to add the molecular weight marker. Can you still analyze the gel and use the results?

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Answer #1

1. Strictly speaking it is always better to run the molecular weight marker when you run the PCR gel.

2. If we have run a positive control for the gene of interest that we are looking in our sample, it will be easily to interpret whether the PCR is worked for our sample.

3. If there are multiple bands in our sample, then it is very difficult to interpret the result as we have not loaded the molecular weight marker.

4. WHen it comes for publication then it is compulsory to take the gel picture with molecular weight ladder to prove that our gene is of particular size which is matching with the ladder.

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