Question

Which way does the dna migrate on a agarose gel electrophoresis and why?

Answer 1

Gel electroforesis technique to separate molecules on the basis of their size and charge.

Agarose gel electrophoresis is used for the separation of DNA fragments and sodium dodecyl sulfate polyacrylamide gel electrophoresis is used for separation of proteins.

DNA molecules have a net negative charge because of the presence of negative phosphate group. Therefore they move towards positive anode during electrophoresis. The agarose gel acts as a molecular sieve through which DNA molecules migrate according to their size. Smaller the size of the fragments, faster is the movement of the fragment and farthest it appears from the loading well.

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