Question

Explain the steps with DNA sequencing and what happens in each step?

Answer 1

DNA sequencing is used to identify the nucleic acid sequence.

The steps are-

1)- Sample collection or DNA extraction - it is done with the use of enzyme like lipase , protease and other enzymes so that only nucleic acid is left undigested.

2)- PCR amplification - the nucleic acid (DNA) is used to amplify the required genetic material.

3)- impurities purification - such as RNA primer etc

4)- sequencing pre preparation - adapter DNA sequence ligated and amplification is done

5)- DNA sequencing - use of sequencer machine helps in making the sequence of required genetic material .

6)- Daya analysis- the sequence is stored in a computer and matched with normal known DNA and mutation are found . This last step comes under bioinformatics.