Question

9. Let’s suppose you had a sample of chromosomal material, but you did not know if it was from a bacterium or a eukaryote. What experiment(s) would you perform to distinguish between bacterial and eukaryotic chromosomes and what would be your expected results?

Answer 1

We would have performed DNA loop technique in which DNA of given chromosome would be hybridized with it's corresponding RNA and if in between loops would have been observed then the chromosomal material was of eukaryote because EK have Introns in their gene and eukaryotic RNA do not contain introns , it get spliced out during transcription so, DNA segment containing intronic region when hybridized with RNA then , the region complementary to RNA will hybridize but Intronic region present in DNA do not have any complementary a sequence in RNA so, it will loop out.

Bacterial Chromosome do not contain Introns, so there will be complete hybridization if the choromosomal material was from a bacterium.