Question

State 7 or more differences or similarieies with the dark field and (vs) florence microscope

Answer 1

Differences:

Fluorescence microscope:

1. emission of light by fluorescent molecule

2. molecules are labelled with fluorescent dyes

3. Can see lived cells

4. Fixed cells

5. look bright and coloured, colourof fluorescent dye

6. for organism identity

Dark-field microscope:

1. principle based on scattering of light

2. relatively thin specimen is required

3. yes can see lived cells

4. Also fixed cells

5. appear brighter or lighted in an otherwise dark field

6. microroganism that exhibit characteristics morphological feature in livin state like in spirochetes