Question

Acetyl coA is made from pyruvate in the ["mitochondrial matrix" or "cytoplasm"] by ["pyruvate dehydrogenase" or "succinate dehydrogenase"] complex. In this process, the acetyl group is moved between enzymes within the complex by the ["lipoamide" or "TPP"]. The ["dihydrolipoyl transacetylase" or "pyruvate dehydrogenase component"] is the enzyme in which acetyl coA is actually formed. The ["FADH2" or "lipoamide"] in the dihydrolipoyl dehydrogenase donates two electrons to NADH, which then goes on to the electron transport chain.

Answer 1

Acetyl-CoA can be generated either by the oxidative decarboxylation of pyruvate from glycolysis, which occurs in mitochondrial matrix, or by the oxidation of long-chain fatty acids, or by oxidative degradation of certain amino acids. Acetyl-CoA then enters in the TCA cycle where it is oxidized for energy production. it is catalysed by the pyruvate dehydrogenase complex. In this process, the acetyl group is moved between enzymes within the complex by the lipoamide. The dihydrolipoyl transacetylase is the enzyme in which acetyl coA is actually formed. The FADH2 in the dihydrolipoyl dehydrogenase donates two electrons to NADH, which then goes on to the electron transport chain.