Question

(i) cell passaging and (ii) the effect of detergents on cells. Connect these subjects for a coherent introduction. Among the things to describe is the general utility of doing cell culture work for cell biology experimentation, the function of fibroblasts within the human body, and the chemical nature of detergents.

Answer 1

I) when cell becomes CONFLUENT then it can no longer be proliferated and thus it have to be passed into another plate which is empty to maintain the Division of cell culture and remain the cell unaffected by contact inhibitions .

2) detergent is a metal salt of acid it disrupt the hydrophobic interactions and thus it can be used to rupture the cells if needed to perform many experiments.

Fibroblast play a critical role in wound healing like it secretes extracellular matrix composites and makes stroma.