Question

You are trying to recreate the experiments to show “spontaneous generation” does not occur. But, after gently boiling, your flasks do have bacterial growth in a few hours even without exposing the broth to air. What is the most likely reason for this?

Group of answer choices

viral contamination and growth

extremely heat stable prions in the media

bacteria evolved to migrate through the swan neck in the flasks

the presence of endospores in the media

spontaneous generation does occur!

Answer 1

The correct answer is option d- the presence of endospores in the media

Explanation: Endospores are heat resistant and they can survive gentle heating. Endospores do not die in the medium when it is heated gently and in the later time, from these endospores, again new bacteria are growing. To kill bacterial endospores autoclaving is necessary where high temperature (121⁰C) and pressure was applied for 15 minutes to completely kill all type of bacterial cells including endospores.