Question

1.   Pyruvate decarboxylase uses as cofactor
   1. Mn²⁺                b. Fe²⁺                          c. Zn²⁺             d. Mg²⁺

                                                            

2. The number of hydrolases involved in the pentose phosphate pathway is  
   1. 1                      b. 2                         c. 3                  d. 0

                       

3.    Substrate-based phosphorylation is involved with the enzyme
   1. aconitase
   2. succinyl-CoA synthetase
   3. citrate synthase
   4. fumarase

4.   The number of carbons in glutaric acid is
   1. 2                      b. 3                         c. 4                  d. 5

5.   The only compound that can’t be produced reversibly in Krebs cycle is
   1. Succinate                    c. malate
   2. Fumarate                     d. a-ketoglutarate

6.    Conversion of succinyl-CoA to oxaloacetate in Krebs cycle involves the following sequence of enzymes
   1. synthetase-dehydrogenase-fumarase-dehydrogenase
   2. synthetase-fumarase-dehydrogenase-dehydrogenase
   3. dehydrogenase-fumarase-synthetase-dehydrogenase
   4. fumarase-dehydrogenase-synthetase-dehydrogenase

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Answer 1

1)a.Mn2+

2)a.1

6-phospho gluco lactone is hydrolyzed to form 6-phospho gluconic acid.

3)b.succinyl CoA synthetase.

Substrate level phosphorylation occurs when succinyl CoA is converted to succinate. (Catalyzed by succinate thiokinase).

4)d.5

Glutaric acid is 5 carbon dicarboxyllic acid.

5)d.alpha keto glutarate

There are 3 irreversible step in Krebs 3 ,catalyzed by isocitrate dehydrogenase (form alpha ketoglutarate),citrate synthase (form citrate),alpha ketoglutarate dehydrogenase (form succinyl CoA).

6)a.synthetase-dehydrogenase-fumarase-dehydrogenase

The enzymes involved sequentially are,succinate thiokinase,succinate dehydrogenase ,fumarase,malate dehydrogenase.