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a student performed negative staining on an unknown culture and noticed coccus shaped cells arranged in...

a student performed negative staining on an unknown culture and noticed coccus shaped cells arranged in pairs and in tetrads. what bacterial species could this be?

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Negative staining only gives the outline of the cells since the cell wall does not bind to the acidic stain (Nigrosin) that is used in this process. On a dark backdrop, the cell shape is identified and if the cells are spherical in shape they belong to the cocci group. Cocci may be arranged single, or in a pair of 2, a pair of 4 (tetrads), cuboidally or randomly as a group of cells. Since they were arranged as a tetrad, they may be belonging to the genus Aerococcus and the species A. viridans

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