Why might phylogeny.fr limit MrBayes phylogenies to "only" 30 sequences?
To perform bayesian inference, Mrbayes software is used for phylogenic reconstruction. It is based on posterior distribution of trees , which is the probability of tree conditioned on the observation. Probability distribution of tree often impossible to calculate analytically. Therefore , Mr bayes used a simulation technique called Markov chain Monte Carlo to calculate posterior probability of tree. There is more time needed for markov chain analysis to converge thats why not suitable for databases with more than 100 sequence. Thats it limit 30 sequence for reliable result in appropriate time.
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